Journal: Drug Design, Development and Therapy

Article Title: Sinomenine Inhibits Orthodontic Tooth Movement and Root Resorption in Rats and Enhances Osteogenic Differentiation of PDLSCs

doi: 10.2147/DDDT.S379468

Figure Lengend Snippet: Results of immunohistochemical staining. ( A – B ) The result of TNF-α ( A ) and RANKL ( B ) immunostaining of the maxillary first molar indicated that the expression of TNF-α and RANKL decreased in sinomenine-treated groups. ( C – E ) The results of OPG ( C ), RUNX2 ( D ) and OCN ( E ) IHC staining indicated that the expression of OPG, RUNX2 and OCN increased. Scale Bar: 20 μm. Data are expressed as mean ± SD (* P<0.05, **P<0.01, ***P<0.001).

Article Snippet: The blots were probed with primary antibodies overnight at 4 °C to ALP (Huabio, Hangzhou, China), RUNX2 (Proteintech, Hubei, China), OPG (Abcam, Cambridge, UK), RANKL and GAPDH (Proteintech, Hubei, China).

Techniques: Immunohistochemical staining, Staining, Immunostaining, Expressing, Immunohistochemistry

Journal: Drug Design, Development and Therapy

Article Title: Sinomenine Inhibits Orthodontic Tooth Movement and Root Resorption in Rats and Enhances Osteogenic Differentiation of PDLSCs

doi: 10.2147/DDDT.S379468

Figure Lengend Snippet: Effect of sinomenine on proliferation and osteogenesis of PDLSCs. ( A ) PDLSCs were treated with 0, 0.1 M, 0.2 M, 0.5 M, 1.0 M, 2.0 M sinomenine for 0, 1, 3, and 5 days, and the growth curves were depicted according to the results of CCK-8 assay. ( B ) 0.1 M sinomenine stimulated the proliferation of PDLSCs, while 1.0 M and 2.0 M sinomenine markedly inhibited the proliferation of PDLSCs on day 5. ( C ) Mineral deposition was observed by Alizarin red staining. Scale bar: 200 μm. ( D ) The relative amount of mineralized nodule was assessed based on the absorbance at 562 nm. ( E ) ALP activity was enhanced by 0.1 M and 0.5 M sinomenine at day 7. ( F and G ) The mRNA and protein expression levels of ALP ( F ) and RUNX2 ( G ) in sinomenine-treated groups for 7 days. Data are presented as mean ± SD (**P<0.01, ***P<0.001, ****P<0.0001).

Article Snippet: The blots were probed with primary antibodies overnight at 4 °C to ALP (Huabio, Hangzhou, China), RUNX2 (Proteintech, Hubei, China), OPG (Abcam, Cambridge, UK), RANKL and GAPDH (Proteintech, Hubei, China).

Techniques: CCK-8 Assay, Staining, Activity Assay, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Depletion of mmu-miR-185 enhances osteocyte connectivity and suppresses bone fragility through the interaction between ARL8B and RAB5A

doi: 10.1186/s13287-025-04612-y

Figure Lengend Snippet: Depletion of miR-185 in MLO-Y4 cells reduces the osteoclastogenic potential of RAW264.7 cells. ( A ) The levels of soluble RANKL (sRANKL) and osteoprotegerin (OPG) in the serum of WT and miR-185 KO mice were quantified by ELISA ( n = 3). ( B ) The concentrations of sRANKL and OPG in conditioned medium from WT and miR-185 KO MLO-Y4 cells were measured by ELISA ( n = 3). ( C ) RAW264.7 cells were co-cultured for 4 days with conditioned medium derived from miR-185 KO, sh Arl8b , and corresponding control MLO-Y4 cells, supplemented with 50 ng/mL RANKL and 30 ng/mL M-CSF ( n = 3). After 4 days of culture, qPCR was performed to evaluate mRNA expression levels of osteoclast marker genes, including Trap , DC-stamp , Ctsk , and Nfatc1 . ( D ) Representative images of tartrate-resistant acid phosphatase (TRAP)-stained osteoclast cultures ( n = 3). ( E ) TRAP-positive cells were counted based on the number of nuclei. ( F ) TRAP-positive multinucleated cells (containing > 3 nuclei) were quantified. ( G ) Cathepsin K (CTSK), a marker of osteoclasts, was detected by western blotting ( n = 3). ( H ) The mRNA expression of matrix metalloproteinase 14 ( Mmp14 ) in MLO-Y4 cells was measured by RT-qPCR ( n = 3). ( I , J ) The protein levels of MMP14 in MLO-Y4 cells were evaluated by western blotting. ( K ) Quantitative analysis of MMP14 protein expression was performed using ImageJ software. ( L )The mRNA expression of Mmp14 in the femurs of mice was assessed by qPCR ( n = 3). Data represent at least three independent experiments and are shown as mean ± S.D. (ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001). The full-length blot is presented in supplementary Raw Western

Article Snippet: RANKL and OPG protein levels in the supernatants were determined using the sRANKL ELISA Kit (CUSABIO, cat# CSB-E05127m) and the OPG ELISA Kit (CUSABIO, cat# CSB-E04693m), respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Derivative Assay, Control, Expressing, Marker, Staining, Western Blot, Quantitative RT-PCR, Software

Journal: Frontiers in endocrinology

Article Title: Modified Qing' e Pills exerts anti-osteoporosis effects and prevents bone loss by enhancing type H blood vessel formation.

doi: 10.3389/fendo.2022.998971

Figure Lengend Snippet: FIGURE 3 MQEP exerts an ameliorative effect on bone turnover regulatory factors and bone structure. (A) Micro-CT was used to evaluate the femur microstructure. The bone structure was observed from three perspectives and reconstructed in three dimensions. (B) Micro-structure measurements were calibrated to the manufacturer’s calcium hydroxyapatite phantom and analyzed using the manufacturer’s evaluation software. (C) Collection of serum and detection of relevant indicators using Elisa. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Concentrations of RANKL and OPG in mice in femur was measured using a commercial mouse RANKL or OPG ELISA kit (Elabscience, Wuhan, China). the femurs were minced into small pieces and rinsed in ice-cold DPBS and weighed, then homogenized in PBS (1:9) with a homogenizer (Servicebio, Wuhan, China).

Techniques: Micro-CT, Software, Enzyme-linked Immunosorbent Assay